Please use this identifier to cite or link to this item:
Title: Platelet Role In Sterile Inflammation
Author: Slaba, Ingrid
Advisor: Kubes, Paul
Keywords: Medicine and Surgery
Issue Date: 23-Dec-2015
Abstract: Platelet responses to focal hepatic necrosis were studied using spinning disk confocal intravital microscopy. Platelets instantly adhered to molecularly altered sinusoidal endothelium adjacent to the necrosis paving approximately 200 µm of vessels abutting the injury. Platelets remained accumulated around the necrosis for at least 4 hours but dissipated by 8 hours. GPIIbIIIa was the main adhesive mechanism; GPIb and GPVI were involved at later stages. Platelets did not cause by-stander injury and did not occlude the vessels. Endothelin-induced vasoconstriction via hepatic stellate cells and not the platelet accumulation or coagulation was responsible for temporarily restricted perfusion around the injury. Neutrophil recruitment over four hours to the sterile necrosis was platelet and GPIIbIIIa dependent. As platelets slowly dissipated neutrophil recruitment was also halted. Healing was delayed in GPIIbIIIa deficient mice. We conclude that in localized sterile injury platelets are essential for neutrophil recruitment and subsequent repair of the lesion.
Appears in Collections:Electronic Theses

Files in This Item:
File Description SizeFormat 
ucalgary_2015_slaba_ingrid_video1.movAccompanying video1.07 MBVideo QuicktimeView/Open
ucalgary_2015_slaba_ingrid_video2.movAccompanying video201.79 kBVideo QuicktimeView/Open
ucalgary_2015_slaba_ingrid_video3.movAccompanying video329.92 kBVideo QuicktimeView/Open
ucalgary_2015_slaba_ingrid_video4.movAccompanying video354.05 kBVideo QuicktimeView/Open
ucalgary_2015_slaba_ingrid_video5.movAccompanying video1.03 MBVideo QuicktimeView/Open
ucalgary_2015_slaba_ingrid_video6.movAccompanying video791.63 kBVideo QuicktimeView/Open
ucalgary_2015_slaba_ingrid_video7.movAccompanying video494.85 kBVideo QuicktimeView/Open
ucalgary_2015_slaba_ingrid_video8.movAccompanying video1.05 MBVideo QuicktimeView/Open
ucalgary_2015_slaba_ingrid_video9.movAccompanying video5.59 MBVideo QuicktimeView/Open
ucalgary_2015_slaba_ingrid.pdfMain document3.6 MBAdobe PDFView/Open

Items in The Vault are protected by copyright, with all rights reserved, unless otherwise indicated.